a simple and specific stability- indicating rp-hplc method for routine assay of adefovir dipivoxil in bulk and tablet dosage form

نویسندگان

bahar darsazan shahid beheshti university of medical sciences

alireza shafaati shahid beheshti university of medical sciences

seyed alireza mortazavi shahid beheshti university of medical sciences

afshin zarghi shahid beheshti univ. med. sci.

چکیده

a simple and reliable stability-indicating rp-hplc method was developed and validated for analysis of adefovir dipivoxil (adv).the chromatographic separation was performed on a c18 column using a mixture of acetonitrile-citrate buffer (10 mm at ph 5.2) 36:64 (%v/v) as mobile phase, at a flow rate of 1.5 ml/min. detection was carried out at 260 nm and a sharp peak was obtained for adv at a retention time of 5.8 ± 0.01 min. no interferences were observed from its stress degradation products. the method was validated according to the international guidelines. linear regression analysis of data for the calibration plot showed a linear relationship between peak area and concentration over the range of 0.5–16 μg/ml; the regression coefficient was 0.9999and the linear regression equation was y=24844x–2941.3. the detection (lod) and quantification (loq) limits were 0.12 and 0.35 μg/ml, respectively. the results proved the method was fast (analysis time less than 7 min), precise, reproducible, and accurate for analysis of adv over a wide range of concentration. the proposed specific method was used for routine quantification of adv in pharmaceutical bulk and a tablet dosage form.

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A Simple and Specific Stability- Indicating RP-HPLC Method for Routine Assay of Adefovir Dipivoxil in Bulk and Tablet Dosage Form

A simple and reliable stability-indicating RP-HPLC method was developed and validated for analysis of adefovir dipivoxil (ADV).The chromatographic separation was performed on a C18 column using a mixture of acetonitrile-citrate buffer (10 mM at pH 5.2) 36:64 (%v/v) as mobile phase, at a flow rate of 1.5 ml/min. Detection was carried out at 260 nm and a sharp peak was obtained for ADV at a reten...

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A Simple and Specific Stability- Indicating RP-HPLC Method for Routine Assay of Adefovir Dipivoxil in Bulk and Tablet Dosage Form

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A Simple and Specific Stability- Indicating RP-HPLC Method for Routine Assay of Adefovir Dipivoxil in Bulk and Tablet Dosage Form

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عنوان ژورنال:
iranian journal of pharmaceutical research

جلد ۱۶، شماره ۱، صفحات ۱۳۲-۱۳۹

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